| Description: |
The Advanced Proteomics Facility provides access to the latest proteomics tools, specifically cutting edge mass spectrometry. It is available to researchers in the South Parks Road science area and to external commercial users.
They have experience in protein identification, quantitative mass spectrometry (SILAC, label-free, dimethyl labelling, TMT) and characterisation methods for a range of post-translational modifications.
The facility is equipped with a wide range of mass spectrometry instrumentation, sample preparation technologies and data analysis software.
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Mass Spectrometers and Sample Preparation Equipment
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LTQ XL (tens of proteins identified per hour). Fixed setup for rapid analysis, most likely one sample every 35 to 45 minutes.
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Orbitrap Elite and a number of QExactives (thousands of proteins identified per hour). These instruments are optimised for state of the art analyses. Single analysis will be anything from 45 minutes to 4 hours.
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HPLC systems to allow fractionation of samples for comprehensive proteome coverage.
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Bioinformatics Software
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Mascot Server (protein identification).
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Proteome Discover 1.4/2.0 (protein identification and quantitation).
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PEAKS (de novo sequencing and post translational modifications).
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Skyline and Pinpoint (targeted quantitation).
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Progenesis label free quantitation software.
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MaxQuant protein identification and quantification software.
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A number of open source software packages.
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