| Description: |
The KIR flow facility operates 2 BD analysers (LSRII, Fortessa X20), 1 Cytek spectral analyser (Aurora), and 1 BD cell sorter (Aria III).
Equipment
- LSR II (R/B/V), Fortessa X20 (R/B/V/YG) are 14-colour maximum panel size. The YG laser on the Fortessa offers an advantage with dsRed/mCherry/tdTomato reporters. The Fortessa has a plate loader.
- Aurora (R/B/V/YG/UV) is a spectral cytometer capable of a 40-colour panel size, with a very effective plate loader for automation. A major part of the KIR scientific remit is immune/stromal crosstalk. This is facilitated by autofluorescence extraction available on the Aurora, whilst the large panel size enables multiplexing of several smaller panels.
- Aria III (R/B/V/YG), configuration equivalent to the Fortessa. Cell sorter is enclosed in a BSC for safe sorting of unscreened blood. Can sort 4 populations simultaneously or into single wells of a 96-well plate. Maximum throughput around 50 million cells/hr for PBMC/splenocyte or similar.
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